Journal: Glycobiology

Article Title: Comparison of biological activities of human antithrombins with high-mannose or complex-type nonfucosylated N-linked oligosaccharides

doi: 10.1093/glycob/cww001

Figure Lengend Snippet: The anticoagulant responses of the purified rhAT-Com in human plasma. Human AT samples were mixed with human plasma prepared from healthy volunteers and heparin at a final concentration of 0 U/mL (open circle), 1 U/mL (closed diamond), 2 U/mL (open square) and 3 U/mL (closed circle) for PT ( A – C ) and 0 U/mL (open triangle), 0.2 U/mL (closed triangle), and 0.4 U/mL (closed square) for APTT ( D – F ). The PT and APPT were measured by an automated coagulation analyzer (Sysmex CA-5000). The data shown are the means ± SE of nine experiments. The PT of phAT (A), rhAT-Comα (B), rhAT-Comβ (C) and the APTT of phAT (D), rhAT-Comα (E) and rhAT-Comβ (F) are shown.

Article Snippet: The anticoagulant activities, including APTT and PT in human plasma, of the rhATs were measured using an automated coagulation analyzer (Sysmex CA-5000; Sysmex, Hyogo, Japan) according to the procedures recommended by the manufacturer.

Techniques: Purification, Clinical Proteomics, Concentration Assay, Coagulation

Journal: Glycobiology

Article Title: Comparison of biological activities of human antithrombins with high-mannose or complex-type nonfucosylated N-linked oligosaccharides

doi: 10.1093/glycob/cww001

Figure Lengend Snippet: The anticoagulant responses of the purified rhAT-Man in human plasma. Human AT samples were mixed with human plasma prepared from healthy volunteers and heparin at a final concentration of 0 U/mL (open circle), 1 U/mL (closed diamond), 2 U/mL (open square) and 3 U/mL (closed circle) for PT ( A – C ) and 0 U/mL (open triangle), 0.2 U/mL (closed triangle) and 0.4 U/mL (closed square) for APTT ( D – F ). The PT and APPT were measured by an automated coagulation analyzer (Sysmex CA-5000). The data shown are the means ± SE of five experiments. The PT of phAT (A), rhAT-Manα (B), rhAT-Manβ (C) and the APTT of phAT (D), rhAT-Manα (E) and rhAT-Manβ (F) are shown.

Article Snippet: The anticoagulant activities, including APTT and PT in human plasma, of the rhATs were measured using an automated coagulation analyzer (Sysmex CA-5000; Sysmex, Hyogo, Japan) according to the procedures recommended by the manufacturer.

Techniques: Purification, Clinical Proteomics, Concentration Assay, Coagulation

Journal: Experimental and Therapeutic Medicine

Article Title: Nicorandil effects on platelet function, Hs-CRP, MMP-9 and myocardial antioxidation in patients with unstable angina

doi: 10.3892/etm.2019.7918

Figure Lengend Snippet: Comparison of R, K and CI in thromboelastogram between the two groups of patients. R and K in experimental group were lower than those in control group and CI in the experimental group was higher than that in control group (*P<0.05). R, coagulation response time; K, formation time of coagulation block; CI, comprehensive coagulation index.

Article Snippet: Moreover, parameters in thromboelastogram, such as coagulation response time (R), formation time of coagulation block (K), thrombosis rate (Angle), comprehensive coagulation index (CI) and maximum amplitude (MA), were measured using a coagulation analyzer (TEG-5000, Haemonetics).

Techniques: Comparison, Control, Coagulation, Blocking Assay